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elisa kits  (Elabscience Biotechnology)


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    Elabscience Biotechnology elisa kits
    Elisa Kits, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+il+17a+elisa+kit/pm41813816-189-20-34?v=Elabscience+Biotechnology
    Average 94 stars, based on 22 article reviews
    elisa kits - by Bioz Stars, 2026-07
    94/100 stars

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    PD-L1-Fc treatment affects the differentiation of uterine T helper cells in mice. (a) qPCR of the expression of IL-17a, IFn-γ, and Il-10 mRNA in the different groups. P values were calculated from one-way ANOVA followed by Tukey’s post hoc test. * P < 0.05. (b) The frequencies of CD4 + T cells producing IFN-γ or IL-17A in the decidua were determined by flow cytometry after in vitro restimulation with PMA and ionomycin. The percentages of IFN-γ+ and IL-17A + T cells are presented as the means ± s.d. of 4–6 individual mice per group. Flow cytometry gating was performed using a standardized workflow (lymphocyte → singlets → CD4 + → cytokine + ). Although representative plots were not captured for publication in this study, all analyses were conducted using identical gating parameters across groups and validated by two independent investigators. (c) After restimulation of sorted CD4 T cells (5 × 10 4 ) with anti-CD3/CD28 monoclonal antibodies (mAbs) for 24 h, the levels of IFN-γ, IL-17A or IL-10 in the cell culture supernatants were determined using ELISA. *p < 0.05; **p < 0.01 by one-way analysis of variance followed by Bonferroni correction. The data are representative of three independent experiments.

    Journal: Frontiers in Pharmacology

    Article Title: Protective effects of an Fc-engineered PD-L1 fusion protein in a spontaneous abortion model and a Th17 cell–induced pregnancy loss model

    doi: 10.3389/fphar.2025.1703805

    Figure Lengend Snippet: PD-L1-Fc treatment affects the differentiation of uterine T helper cells in mice. (a) qPCR of the expression of IL-17a, IFn-γ, and Il-10 mRNA in the different groups. P values were calculated from one-way ANOVA followed by Tukey’s post hoc test. * P < 0.05. (b) The frequencies of CD4 + T cells producing IFN-γ or IL-17A in the decidua were determined by flow cytometry after in vitro restimulation with PMA and ionomycin. The percentages of IFN-γ+ and IL-17A + T cells are presented as the means ± s.d. of 4–6 individual mice per group. Flow cytometry gating was performed using a standardized workflow (lymphocyte → singlets → CD4 + → cytokine + ). Although representative plots were not captured for publication in this study, all analyses were conducted using identical gating parameters across groups and validated by two independent investigators. (c) After restimulation of sorted CD4 T cells (5 × 10 4 ) with anti-CD3/CD28 monoclonal antibodies (mAbs) for 24 h, the levels of IFN-γ, IL-17A or IL-10 in the cell culture supernatants were determined using ELISA. *p < 0.05; **p < 0.01 by one-way analysis of variance followed by Bonferroni correction. The data are representative of three independent experiments.

    Article Snippet: ELISA kits for IFN-γ, IL-17A, and IL-10 (R&D Systems; cat. nos.

    Techniques: Expressing, Flow Cytometry, In Vitro, Bioprocessing, Cell Culture, Enzyme-linked Immunosorbent Assay